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The RNA-seq data have been deposited in the NCBI Sequence Read Archive (SRA) (SRP133455). Uncropped western blots are provided as Supplementary Fig. 1. All other data that support the findings of this study can be found as in bioRxiv preprint11 or are available from the corresponding authors upon reasonable request.
Source code are available upon request.
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This study is supported by US National Institutes of Health grant AG047200 to V.G. and A.S., and Natural Science Foundation of China grant 81672818, Science Technology and Innovation Committee of Shenzhen Municipality grant JCYJ20160331190123578 and Guangzhou Science and Technology Program key project 201604020005 to X.F.
The authors declare no competing interests.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data figures and tables
Extended Data Fig. 1 Tumorigenicity of the cell lines and PCA of the RNA-seq datasets.
a, Tumorigenicity of the cell lines. Cells not analysed in this study are indicated with a ‘-’. All transforming genes were driven by a retroviral LTR promoter. b–e, PCA of the RNA-seq datasets. PCA was done using the expression of 13,276 orthologous genes across all four species (mouse (b), blind mole rat (c), NMR (d) and human (e)). The first two principal components of each analysis were extracted. Values in parentheses indicate the variance explained by each of the principal components. L, SV40LT; R, HRAS(G12V); T, human TERT. All transgenes are driven by LTR promoters.
Extended Data Fig. 2 Enrichment analysis of DEGs in the transformed cells across species.
a, GO enrichment analysis of the DEGs derived from Fig. 1a. b, GO enrichment analysis of the DEGs derived from Fig. 1b. c, GO enrichment analysis of the DEGs derived from Fig. 1c. Biological process and molecular function terms are shown. All GO terms in a–c are arranged in the order of decreasing significance in the mouse cells. The top 20 significantly upregulated (red) and downregulated (green) terms are shown. LT, SV40LT; RAS, HRAS(G12V). All transgenes are driven by LTR promoters.
This file contains Supplementary Methods and Supplementary Figure 1 showing uncropped Western blot images.
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Zhao, J., Tian, X., Zhu, Y. et al. Reply to: Transformation of naked mole-rat cells. Nature 583, E8–E13 (2020). https://doi.org/10.1038/s41586-020-2411-9
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