a, Protein abundance and localization of two putative EMC clients (Mrh1 and Fet3) in wild-type and EMC3(K26L) mutant yeast strains. The eGFP is appended to the C termini of the genes. b, Growth experiments of yeast strains containing Emc4 linker loop truncations. The three truncations were Emc4(∆56–60), Emc4(∆51–60), and Emc4(∆46–60). Experiments in a and b were repeated three times with similar results.