Extended Data Fig. 2: Haematoxylin and eosin staining and immunohistochemistry on hamster tissues challenged with SARS-CoV-2. | Nature

Extended Data Fig. 2: Haematoxylin and eosin staining and immunohistochemistry on hamster tissues challenged with SARS-CoV-2.

From: Pathogenesis and transmission of SARS-CoV-2 in golden hamsters

Extended Data Fig. 2

a, Hyperplasia of the pneumocytes detected at 7 dpi. b, Detection of CD3-positive cells (using rabbit anti-human CD3 polyclonal antibody) in the lungs at 5 dpi. c, Detection of SARS-CoV-2 N protein (red staining, using monoclonal antibody 4D11) and olfactory neurons (brown staining, using monoclonal antibody TuJ1) from the nasal turbinate at 5 dpi. d, Detection of olfactory neurons (using monoclonal antibody TuJ1) from the nasal turbinate of a mock-infected hamster (n = 1). e, Nasal epithelial cells from the nasal turbinate of a mock-infected hamster (n = 1) showed negative staining for TuJ1. f, Detection of olfactory neurons from nasal turbinate at 2 dpi. g, Detection of olfactory neurons from nasal turbinate at 7 dpi. h, Detection of olfactory neurons from nasal turbinate at 14 dpi. i, Haematoxylin and eosin (H&E) staining of the brain tissue at 5 dpi. j, H&E staining of the heart at 5 dpi. k, H&E staining of the liver at 5 dpi. l, H&E staining of the kidney at 5 dpi. Hamsters were intranasally inoculated with PBS (mock infection, n = 1) or with 8 × 104 TCID50 of SARS-CoV-2 (n = 9), and the tissues were collected at 2 (n = 3), 5 (n = 3) and 7 (n = 3) dpi. H&E and immunohistochemistry performed on tissues from three hamsters at day 2, 5 and 7 dpi showed similar results; representative results are shown.

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