Fig. 1: Viral load and histopathological changes in golden hamsters intranasally challenged with SARS-CoV-2. | Nature

Fig. 1: Viral load and histopathological changes in golden hamsters intranasally challenged with SARS-CoV-2.

From: Pathogenesis and transmission of SARS-CoV-2 in golden hamsters

Fig. 1

a, Infectious viral load (log10(TCID50 per ml)) and viral RNA (log10(RNA copies per ml)) detected in the lungs of hamsters challenged with SARS-CoV-2 (n = 3) at 2, 5 and 7 dpi. b, Infectious viral load and viral RNA detected in the kidney of hamsters challenged with SARS-CoV-2 (n = 3) at 2, 5 and 7 dpi. Individual data points and mean ± s.d. are shown; the detection limit (1.789 log10(TCID50 per ml)) is shown by the dotted line. c, Haematoxylin and eosin (H&E) staining of the lungs of hamsters challenged with SARS-CoV-2 at 2 dpi. d, Detection of SARS-CoV-2 N protein at bronchial epithelial cells (indicated by an arrow) by immunohistochemistry at 2 dpi. e, H&E staining of the lungs at 5 dpi. f, Detection of N protein in pneumocytes with lung consolidation (indicated by an arrow) at 5 dpi. g, H&E staining of the lungs at 7 dpi. h, The lack of detection of N protein in the lungs at 7 dpi. i, H&E staining of nasal turbinate of hamsters challenged with SARS-CoV-2 at 2 dpi. j, Detection of N protein in nasal epithelial cells (arrow on the right) and cells morphologically resembling olfactory neurons (arrow on the left) at 2 dpi. k, H&E staining of duodenum of hamsters challenged with SARS-CoV-2 at 2 dpi. l, Detection of N protein in the duodenum epithelial cells at 2 dpi. The experiment was performed once with 9 hamsters challenged with 8 × 104 TCID50 of SARS-CoV-2, and tissues were collected from 3 hamsters for histopathology examination and immunohistochemistry at each time point. H&E staining and immunohistochemistry performed using tissues from three hamsters showed comparable results; representative images are shown. Scale bars, 200 μm (i, j), 500 μm (ch, k, l).

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