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Each serum sample was tested in triplicate. Serum samples from two healthy individuals from Wuhan and five patients as well as a horse anti-SARS-CoV anti-serum were used. We used 120 TCID50 viruses per well. Serum samples were used at dilutions of 1:10, 1:20, 1:40 and 1:80. neg, negative; VNT, virus neutralization test.