Extended Data Fig. 5: Effect of CD8+ T cell depletion on antibody production and epithelial stress markers. | Nature

Extended Data Fig. 5: Effect of CD8+ T cell depletion on antibody production and epithelial stress markers.

From: IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac disease

Extended Data Fig. 5

af, Ten-week-old DQ8-Dd-villin-IL-15tg mice were treated with 200 μg anti-CD8α antibody (clone 2.43) or its isotype control (rat IgG2b) twice before and during the course of gluten feeding. a, Experimental scheme. b, c, Representative dot plots showing depletion efficiency in the blood (b) and epithelium (c) of DQ8-Dd-villin-IL-15tg mice after 30 days of anti-CD8α treatment. d, e, Expression of Rae1 (d) and Qa-1 (e) genes in the intestinal epithelium was determined by qPCR. Relative expression levels were normalized against the expression levels observed in sham-fed DQ8-Dd-villin-IL-15tg mice (gluten + isotype, n = 16; gluten + anti-CD8, n = 14). f, Serum levels of anti-DGP IgG 30 days after gluten feeding (n = 11 mice per group). Data are mean ± s.e.m. (d, e) or mean values (f) from four (be) or three (f) independent experiments. P values determined by unpaired, two-tailed, t-test (df).

Source data

Back to article page