Extended Data Fig. 1: Development of villous atrophy requires IL-15 expression in both the lamina propria and epithelium. | Nature

Extended Data Fig. 1: Development of villous atrophy requires IL-15 expression in both the lamina propria and epithelium.

From: IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac disease

Extended Data Fig. 1

ag, DQ8-Dd-IL-15tg, DQ8-villin-IL-15tg and DQ8-Dd-villin-IL-15tg mice that were raised on a GFD were maintained on a GFD (sham) or fed gluten for 30 days. a, Expression of Ifng in the lamina propria was measured by qPCR (DQ8-Dd-IL-15tg sham, n = 7, gluten n = 16; DQ8-villin-IL-15tg sham, n = 13, gluten n = 12). b, Serum anti-gliadin IgG2c levels were measured by ELISA 30 days after gluten feeding (DQ8-Dd-IL-15tg sham, n = 16, gluten n = 16; DQ8-villin-IL-15tg sham, n = 15, gluten n = 15). c, Serum levels of anti-DGP IgG were measured by ELISA 30 days after gluten feeding (DQ8-Dd-IL-15tg sham, n = 10, gluten n = 10; DQ8-villin-IL-15tg sham, n = 9, gluten n = 9). d, Quantification of IELs among IECs was performed on H&E-stained ileum sections (DQ8-Dd-IL-15tg sham, n = 11, gluten n = 12; DQ8-villin-IL-15tg sham, n = 17, gluten n = 16; DQ8-Dd-villin-IL-15tg sham, n = 14, gluten n = 17). e, Left, H&E staining of paraffin-embedded ileum sections. Right, the ratio of the morphometric assessment of villus height to crypt depth (DQ8-Dd-IL-15tg sham, n = 10, gluten n = 10; DQ8-villin-IL-15tg sham, n = 17, gluten n = 19; DQ8-Dd-villin-IL-15tg sham, n = 21, gluten n = 28). f, g, Expression of Rae1 (f) and Qa-1 (g) in the intestinal epithelium was measured by qPCR. Relative expression levels in sham and gluten-fed mice for each strain are shown (DQ8-Dd-IL-15tg sham, n = 16, gluten n = 16; DQ8-villin-IL-15tg sham, n = 15, gluten n = 15; DQ8-Dd-villin-IL-15tg sham, n = 20, gluten n = 26). Data are mean ± s.e.m. (a, f, g) or mean values (be) from three (ac, f, g), four (d) or six (e) independent experiments. P values were determined by one-way ANOVA with Tukey’s multiple comparison test (ae) or unpaired, two-tailed, t-test (f, g).

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