Extended Data Fig. 9: Effect of IFNγ and IL-21 neutralization on the development of coeliac disease. | Nature

Extended Data Fig. 9: Effect of IFNγ and IL-21 neutralization on the development of coeliac disease.

From: IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac disease

Extended Data Fig. 9

ai, DQ8-Dd-villin-IL-15tg mice were treated with 500 μg of anti-IFNγ (clone XMG1.2) and/or anti-IL21R (clone 4A9) antibodies or corresponding isotype controls (rat IgG1 and rat IgG2a, respectively) once before, and every 3 days during the course of gluten feeding as indicated. a, Ratio of the morphometric assessment of villus height to crypt depth (sham, n = 8, gluten + isotype, n = 7, gluten + anti-IL-21R, n = 6). b, Quantification of IELs among IECs was performed on H&E-stained ileum sections (sham, n = 8, gluten + isotype, n = 7, gluten + anti-IL-21R, n = 6). c, The intestinal epithelium was isolated and analysed by flow cytometry. IELs were identified as TCRβ+CD4CD8+ cells. Granzyme B+ IELs are indicated by absolute number per 100 IECs (sham, n = 8, gluten + isotype, n = 7, gluten + anti-IL-21R, n = 6). d, Granzyme B+ IELs are indicated as in c (sham, n = 12, gluten + isotype, n = 16, gluten + anti-IL-21R + anti- IFNγ, n = 11). e, Quantification of IELs among IECs was performed on H&E-stained ileum sections (sham, n = 13, gluten + isotype, n = 17, gluten + anti-IL-21R+ anti-IFNγ, n = 11). f, g, Serum levels of anti-DGP IgG (f) and anti-gliadin IgG2c (g) antibodies were measured by ELISA. Serum was collected 30 days after gluten feeding (sham, n = 5 and 6, gluten + isotype, n = 8 and 7, gluten + anti-IL-21R, n = 6). h, i, Serum levels of anti-DGP IgG (h) and anti-gliadin IgG2c (i) antibodies were measured as in f and g (sham, n = 6 and 6, gluten + isotype, n = 8, gluten + anti-IFNγ, n = 6). Data are mean values (ai) from two (ac, fi) or four (d, e) independent experiments. P values determined by ANOVA with Tukey’s multiple comparison test (ai).

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