Extended Data Fig. 4: Complementation experiments performed with small DNA or antisense sRNA do not promote repair. | Nature

Extended Data Fig. 4: Complementation experiments performed with small DNA or antisense sRNA do not promote repair.

From: BRCA1 and RNAi factors promote repair mediated by small RNAs and PALB2–RAD52

Extended Data Fig. 4

a, BELD quantification of breaks on the forward (coding) strand of the CASP16P pause2 termination site in BRCA1-depleted HeLa cells complemented with either ACTB or CASP16P sdRNA, showing that only CASP16P sdRNA can prevent the accumulation of breaks at the CASP16P pause2 site. A representative experiment is shown, and the histograms depict the mean ± s.d. values of the qPCR replicates. b, c, DNA damage quantification using γ-H2AX ChIP qPCR analyses performed in complementation experiments at the ACTB and CASP16P pause sites and at the CCNB1 CoTC-type terminator. ChIP data are represented as the mean ± s.e.m. fold change compared to an undepleted relevant control. Complementation experiments were performed in BRCA1-depleted (b) or DICER1-depleted (c) HeLa cells reconstituted with the 3′-non-polymerizable sense sdRNA (3′-blocked ACTB sdRNA) (b, c) or ACTB or CASP16P antisense (AS) sdRNA (b, c) or the corresponding sense or antisense sdRNA DNA sequence (b). n = 2–6 biological replicates (b); n = 2–6 biological replicates (c). b, c, Data were analysed by two-way ANOVA with post hoc Tukey HSD test and compared to the relevant control cells.

Back to article page