Extended Data Fig. 10: Overexpression of ACTB or CASP16P sdRNA results in DNA damage at the pause site of the gene that encoded it. | Nature

Extended Data Fig. 10: Overexpression of ACTB or CASP16P sdRNA results in DNA damage at the pause site of the gene that encoded it.

From: BRCA1 and RNAi factors promote repair mediated by small RNAs and PALB2–RAD52

Extended Data Fig. 10

a, DNA damage quantification at ACTB and CASP16P termination pause sites following ACTB or CASP16P sdRNA overexpression (OE) using γ-H2AX ChIP qPCR analyses. The ChIP signal reflects the mean ± s.e.m. fold change over the untransfected condition (control). n = 4–6 biological replicates. Data were analysed by two-way ANOVA with post hoc Tukey HSD test and compared to the relevant control cells. b, Model of sdRNA-mediated DNA damage repair at R-loop-positive pause sites in proliferating and quiescent cells.

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