Extended Data Fig. 9: PALB2 and RAD52 depletion induce DNA damage in proliferating T98G and validation of efficient G0 arrest upon PALB2 and RAD52 depletion. | Nature

Extended Data Fig. 9: PALB2 and RAD52 depletion induce DNA damage in proliferating T98G and validation of efficient G0 arrest upon PALB2 and RAD52 depletion.

From: BRCA1 and RNAi factors promote repair mediated by small RNAs and PALB2–RAD52

Extended Data Fig. 9

a, γ-H2AX ChIP quantification analysed in proliferating BRCA1-, PALB2- or RAD52-depleted T98G cells (n = 3 biological replicates). ChIP results are shown as the mean ± s.e.m. fold change compared to the mock siRNA. b, Representative immunoblots showing the efficiency of siRNA-mediated depletion of PALB2 and RAD52 in both quiescent/G0 arrested and asynchronous T98G cells. Representative blot from n = 4 experiments. p27 is a marker of quiescence in this setting.

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