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Worms were sorted onto NGM plates and assessed for lifespan. P values for Kaplan–Meier survival analysis were calculated on the basis of the log-rank (Mantel–Cox) method unless noted otherwise. Samples were compared with L2ox subpopulations. Maximum lifespan was defined as the lifespan of the last 10% of the population. E.V., empty vector (control RNAi conditions for experiments in Extended Data Table 4).
*Percentage change based on mean lifespan.
†Percentage change based on maximum lifespan.
‡P value for survival analysis calculated based on the Gehan–Breslow–Wilcoxon method.
§Grx1–roGFP2 ratio of L2ox and L2red subpopulations, based on BioSorter analysis during sorting. Data represent mean ± s.e.m. n, number of worms. Two-sided unpaired t-test.
ǁGrx1–roGFP2 ratio of L2ox and L2red subpopulations based on microscopy analysis, following sorting. Data are mean ± s.e.m. n, number of worms. Two-sided unpaired t-test.
¶Lifespan assay conducted with experimenter blind to the type of worms assayed.