a, Length measurements of L2ox and L2red worms (symbol) from nose to tail tip immediately after sorting. No significant difference; P = 0.4735 (unpaired two-sided t-test). b, Brood size of L2ox and L2red worms, measured at the indicated time points. n, number of worms. No significant difference within a single age; P = 0.6532 (two-way ANOVA). c–f, Basal respiration (c), maximal (d) and spare (e) respiratory capacity and basal rates of flux through glycolysis (f) of L2ox and L2red worms. n = 3 independent sorting experiments. ECAR, extracellular acidification rate; OCR, oxygen consumption rate. P = 0.9469 (c), P = 0.7784 (d), P = 0.7904 (e) and P = 0.7925 (f); two-sided unpaired t-test. g, Survival of L2ox, L2mean and L2red worms 20 h after heat shock. n = 5 independent sorting experiments; two-sided unpaired t-test. The connected data points represent data from the same sorting experiment. The survival of L2ox is set to 1. All data are mean ± s.e.m.