a, Microscopy analysis of the Grx1–roGFP2 ratio of individual N2jrIs2[Prpl-17::Grx1-roGFP2] worms (symbol) cultivated at 15 °C and imaged at the indicated time points. Data points that are not significantly different from each other (P > 0.05) are labelled with the same letter. Data are mean ± s.e.m; n, number of worms; one-way ANOVA with Tukey correction. b, The Grx–roGFP2 ratio (A405/A488) was calculated using the partial profiling feature (pp) configured to analyse extinction and emission data from 488 nm and 405 nm lasers that sequentially excited each worm. c, A population of N2jrIs2[Prpl-17::Grx1-roGFP2] at the L2 stage, separated based on opacity (extinction) and length (time of flight) was gated as R1.