Extended Data Fig. 6: Effects of H3K4me3 downregulation on heat-shock response and endogenous redox state. | Nature

Extended Data Fig. 6: Effects of H3K4me3 downregulation on heat-shock response and endogenous redox state.

From: Developmental ROS individualizes organismal stress resistance and lifespan

Extended Data Fig. 6

a, ASH-2 and SET-2 transcript levels of N2jrIs2[Prpl-17::Grx1-roGFP2] worms treated with ash-2 or set-2 RNAi for 2 generations. n = 6 (ash-2) and n = 2 (set-2) independent experiments; unpaired two-sided t-test. E.V., empty vector. b, ASH-2 protein levels in N2jrIs2[Prpl-17::Grx1-roGFP2] worms treated with control RNAi or ash-2 RNAi for two generations using western blot analysis. c, H3K4me3 levels in N2jrIs2[Prpl-17::Grx1-roGFP2] worms treated with control RNAi, ash-2 RNAi or set-2 RNAi for two generations. d, Transcript levels of selected heat-shock genes after heat-shock treatment of N2jrIs2[Prpl-17::Grx1-roGFP2] worms treated with the indicated RNAi. n = 3 independent experiments; one-way ANOVA with Bonferroni correction. e, Transcript levels of selected heat-shock genes in set-2 or wdr-5.1 mutants before and after heat-shock treatment. n = 3 independent experiments; one-way ANOVA with Bonferroni correction. f, ASH2L levels following ASH2L siRNA treatment of HeLa cells. n = 2 independent experiments. g; Grx1–roGFP2 ratios of L2 larval worms treated with ash-2 RNAi, set-2 RNAi, wdr-5.1 RNAi or the empty vector were measured using the BioSorter. n = 4 (ash-2, set-2) and n = 3 (wdr-5.1) independent sorting experiments; unpaired two-sided t-test. h, Representative survival curves of N2jrIs2[Prpl-17::Grx1-roGFP2] worms treated with ash-2 or set-2 RNAi for two generations and treated with 1 mM paraquat (PQ) for 10 h at the L2 larval stage. For n numbers, repetitions and statistics (log-rank), see Extended Data Table 4. Data in a, dg, represent mean ± s.e.m. For blot source images, see Supplementary Figs. 1, 3.

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