Extended Data Fig. 9: Effects of MLLT3 knockdown and overexpression on CB-HSPC expansion in vitro. | Nature

Extended Data Fig. 9: Effects of MLLT3 knockdown and overexpression on CB-HSPC expansion in vitro.

From: MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment

Extended Data Fig. 9

a, FACS analysis, 5 and 15 days after MLLT3-KD in CB-HSPCs (representative of two experiments). b, Quantification of haematopoietic subsets CD34+CD38−/lo and CD34+CD38−/loCD90+ (HSPCs) in empty vector (CTR) and MLLT3-KD-transduced cells after 5, 15 and 30 days in culture (mean, n = 2). Circles represent individual values. c, FACS analysis of CB-HSPCs transduced with control or MLLT3-OE vectors cultured on OP9M2 (representative of two experiments). d, Expansion of total live cells (left) and CD34+CD38−/lo CD90 HSPCs (right) transduced with MLLT3-OE or control vectors.

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