Extended Data Fig. 7: Fusion of EcoRI restriction enzymes to ORF152 enables immune activity. | Nature

Extended Data Fig. 7: Fusion of EcoRI restriction enzymes to ORF152 enables immune activity.

From: A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases

Extended Data Fig. 7

a, Strain PAO1 was engineered to express EcoRI or an EcoRI–ORF152 fusion protein. Plaque assays were conducted as in Fig. 1a and quantified (n = 3). b, Growth curves measuring the OD600 nm of PAO1 cells that were infected with ΦKZ at the indicated MOI. c, d, Live-cell fluorescence imaging (with a wide field of view in d) of P. aeruginosa strains that were engineered to express EcoRI or EcoRI–ORF152. The DAPI stain shows the phage DNA. All plaque assays were replicated three times with similar results. The growth curve experiments (b) were replicated twice with similar results. Microscopy (c, d) was replicated twice with similar results. Scale bar, 5 μm.

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