Extended Data Fig. 10: FACS schematic for in vitro and in vivo fibroblast analysis and sorting and full western blot membranes. | Nature

Extended Data Fig. 10: FACS schematic for in vitro and in vivo fibroblast analysis and sorting and full western blot membranes.

From: Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing

Extended Data Fig. 10

a, FACS schematic for analysis and sorting of THY1PDGFRα+ and THY1+PDGFRα+ cells in young and old cultures at passage 3. Gates shown on each plot are indicated above the plot. Marker and fluorophore are shown on each axis. FMO, fluorescence minus one. b, FACS schematic for analysis and sorting of live THY1PDGFRα+Lin and THY1+PDGFRα+Lin cells from young and old fresh tissues (ears), used for population RNA-seq and single-cell RNA-seq analyses. Gates shown on each plot are indicated above the plot. Marker and fluorophore are shown on each axis. c, Full western blot membranes from Extended Data Fig. 6d, i, j. Boxes indicate the cropped area.

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