Extended Data Fig. 8: Genetic variation in the human UNC93B1 C-terminal regulatory region increases TLR7 responses. | Nature

Extended Data Fig. 8: Genetic variation in the human UNC93B1 C-terminal regulatory region increases TLR7 responses.

From: UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity

Extended Data Fig. 8

NF-κB activation in human HEK293T cells transiently expressing TLR7 or TLR5 and the indicated human UNC93B1 alleles was measured using a dual luciferase reporter assay. Cells were stimulated with R848 (10 ng ml−1) or flagellin (2 ng ml−1) for 16 h before collection. Data are normalized to Renilla expression and expressed as RLUs. Data are mean ± s.d., n = 3 biological replicates. *P < 0.05, **P < 0.01 and ***P < 0.001, one-way ANOVA followed by a Tukey’s post-test (95% confidence interval). Data are representative of three independent experiments.

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