Extended Data Fig. 6: WNT-related gene activation correlates with loss of p53 in mouse and human breast tumours. | Nature

Extended Data Fig. 6: WNT-related gene activation correlates with loss of p53 in mouse and human breast tumours.

From: Loss of p53 triggers WNT-dependent systemic inflammation to drive breast cancer metastasis

Extended Data Fig. 6

a, b, Heat maps showing that Trp53−/− (KO) GEMM tumours (n = 77) cluster away from Trp53+/+ (WT) tumours (n = 68) based on analysis of the Hallmark p53 pathway (represents positive control) (a) and analysis of the Hallmark WNT and β-catenin pathway (b). Analysis was performed on all tumours of Extended Data Fig. 5a. c, The log-transformed fold change in expression of genes involved in WNT signalling (P < 0.05) in Trp53−/− (n = 77) and Trp53+/+ (n = 68) GEMM tumours depicted in Extended Data Fig. 5a. Black bars indicate genes that positively regulate or are generally increased with active WNT signalling. Red bars indicate genes that negatively regulate or are downregulated with active WNT signalling. d, Gene set enrichment analysis (GSEA) for Hallmark pathways in TCGA wild-type TP53 breast tumours (n = 643) versus mutant TP53 (n = 351) human tumours (any TP53 mutation) or TP53 loss (based on the IARC TP53 database; see Methods). Normalized enrichment score is shown with the FDR indicated. e, Correlation coefficient (R) of all genes involved in WNT signalling that correlate significantly (P < 0.05) with mutant TP53 (n = 351) versus wild-type TP53 (n = 643) in TCGA breast tumours. Black bars indicate genes that positively regulate or are generally increased with active WNT signalling. Red bars indicate genes that negatively regulate or are downregulated with active WNT signalling. P values were determined by two-tailed ANOVA with FDR multiple-testing correction (c, e).

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