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Reply to: Egg pigmentation probably has an Archosaurian origin

The Original Article was published on 19 June 2019

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Fig. 1: Raman spectroscopic and statistical analysis of an eggshell pigmentation versus an egg colour signal.

Data availability

All data supporting the findings of this study are available within the paper (Fig. 1), and its Extended Data (Extended Data Fig. 1, Extended Data Table 1).

References

  1. Shawkey, M. D. & D’Alba, L. Egg pigmentation probably has an Archosaur origin. Nature https://doi.org/10.1038/s41586-019-1282-4 (2019).

    Article  Google Scholar 

  2. Wiemann, J., Yang, T.-R. & Norell, M. A. Dinosaur egg colour had a single evolutionary origin. Nature 563, 555–558 (2018).

    Article  ADS  CAS  Google Scholar 

  3. Kennedy, G. Y. & Vevers, H. G. A survey of avian eggshell pigments. Comp. Biochem. Physiol. B 55, 117–123 (1976).

    Article  CAS  Google Scholar 

  4. Gosler, A. G., Higham, J. P. & Reynolds, S. J. Why are birds’ eggs speckled? Ecol. Lett. 8, 1105–1113 (2005).

    Article  Google Scholar 

  5. Wiemann, J. et al. Dinosaur origin of egg color: oviraptors laid blue-green eggs. PeerJ 5, e3706 (2017).

    Article  Google Scholar 

  6. Salzer, R. & Siesler, H. W. (eds). Infrared and Raman Spectroscopic Imaging (John Wiley & Sons, 2014).

  7. Thomas, D. B. et al. Analysing avian eggshell pigments with Raman spectroscopy. J. Exp. Biol. 218, 2670–2674 (2015).

    Article  Google Scholar 

  8. Wiemann, J. et al. Fossilization transforms vertebrate hard tissue proteins into N-heterocyclic polymers. Nat. Commun. 9, 4741 (2018).

    Article  ADS  Google Scholar 

  9. Mikšík, I., Paradis, S., Eckhardt, A. & Sedmera, D. Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome. Protein J. 37, 21–37 (2018).

    Google Scholar 

  10. O’Reilly, J. E. et al. Bayesian methods outperform parsimony but at the expense of precision in the estimation of phylogeny from discrete morphological data. Biol. Lett. 12, 20160081 (2016).

    Article  Google Scholar 

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Acknowledgements

We thank D. E. G. Briggs for assistance with the manuscript, and M. Fabbri and J. Gauthier for suggestions.

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Contributions

J.W., T.-R.Y. and M.A.N. discussed Shawkey and D’Alba’s concerns. J.W. designed and performed the experiments, analysed the data, and created the figure. J.W. wrote the manuscript, which was reviewed by all authors.

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Correspondence to Jasmina Wiemann.

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The authors declare no competing interests.

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Extended data figures and tables

Extended Data Fig. 1 Crocodylus siamensis outer and inner eggshell surfaces.

a, The outer eggshell surface (n = 1), which contains the highest pigment concentrations in eumaniraptorans is uncoloured/white. b, c, The inner eggshell surface (n = 2) shows a reddish tint (b), which reflects chorioallantoic tissues and vascularity (c) protruding into the innermost layers of the eggshell. The inner eggshell surface in b is manually cleaned of adjacent chorioallantoic tissue, whereas the inner eggshell surface in c is left untreated. Chorioallantoic tissues (c) are commonly vascularized, and therefore saturated in blood-derived haem, which represents, when dechelated, protoporphyrin. In this case, traces of protoporphyrin recovered from Crocodylus siamensis eggshell would not be homologous with eumaniraptoran eggshell protoporphyrin.

Extended Data Table 1 Information on added taxa (n = 4), catalogue numbers, ages, localities, and egg colours

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Wiemann, J., Yang, TR. & Norell, M.A. Reply to: Egg pigmentation probably has an Archosaurian origin. Nature 570, E46–E50 (2019). https://doi.org/10.1038/s41586-019-1283-3

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