Extended Data Fig. 4: Analysis of the association of Cdc48 with mitochondria. | Nature

Extended Data Fig. 4: Analysis of the association of Cdc48 with mitochondria.

From: Mitochondrial protein translocation-associated degradation

Extended Data Fig. 4

a, Crude and purified wild-type and ubx2∆ mitochondria were analysed by SDS–PAGE and immunodetection with the indicated antisera. The data are representative of four independent experiments. b, Cdc48 levels in purified mitochondria from mutant strains of Cdc48-binding proteins19,20,25,42 were analysed by SDS–PAGE and immunodetection. The data are representative of five independent experiments. c, Affinity purifications of Tom40–HA, Tom40–HA Ubx2 ∆UBA and Tom40–HA Ubx2∆UBX cell extracts. The data are representative of three independent experiments. Load 2% (0.1% for Cdc48); elution 100%.

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