Extended Data Fig. 4: DCX–eYFP+progenitors in prostate tumours can proliferate and/or differentiate into neurons ex vivo. | Nature

Extended Data Fig. 4: DCXeYFP+progenitors in prostate tumours can proliferate and/or differentiate into neurons ex vivo.

From: Progenitors from the central nervous system drive neurogenesis in cancer

Extended Data Fig. 4

a, LineYFP+ cells do not display immune or endothelial phenotypes. Scatter plot representations (also known as ImmGen W-plots) of the mean-normalized expression values of the 200 most-highly expressed genes of each sample (SVZ, olfactory bulb or prostate, n = 2 per region) in each of the selected ImmGen cell populations. ImmGen populations are coloured on the basis of their main cell population families, listed in Supplementary Table 5 with correlation coefficients and P values. b, Differentiation of DCXeYFP+ cells from prostate tumour tissues after one or eight days culture in neural medium supplemented with epidermal growth factor and basic fibroblast growth factor (EGF and bFGF, proliferation medium), or brain-derived neurotrophic factor and neurotrophin-3 (BDNF and NT-3, differentiation medium). Scale bar, 400 μm. Two independent experiments. c, Immunostaining of MAP2+ differentiated DCXeYFP+ neural cells isolated from tumour (right) or olfactory bulbs (left). Scale bar, 20 μm. Three independent experiments. d, e, Quantification of proliferation of neural progenitors (d) and differentiated neural cells (e) with one or more neurites (red arrows (for example in b)) for eight days. Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). ***P < 0.001. Two independent experiments.

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