Extended Data Fig. 6: In Hi-MYC mouse model of cancer, Lin−eYFP+ progenitors egress the SVZ, migrate through the blood and infiltrate the tumour, where they do not express neural stem-cell markers. | Nature

Extended Data Fig. 6: In Hi-MYC mouse model of cancer, LineYFP+ progenitors egress the SVZ, migrate through the blood and infiltrate the tumour, where they do not express neural stem-cell markers.

From: Progenitors from the central nervous system drive neurogenesis in cancer

Extended Data Fig. 6

a, b, After tamoxifen-induced recombination in the DCX-creERT2;loxp-eYFP Hi-MYC cancer mice at week 3 after birth, the number of LineYFP + neural progenitors decreases in the SVZ at week 6 after birth (a) and—in turn—LineYFP + cells emerge progressively in blood (b). Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Sample sizes are listed in Source Data. Thirteen independent experiments. c, d, Representative FACS plots of LineYFP+ cells isolated from the SVZ (c) or blood (d) of 6- or 16-week-old wild-type or Hi-MYC mice. ****P < 0.0001. e, LineYFP+SCA-1PSA-NCAM cells in the SVZ—but not in prostate tumours—express neural stem-cell markers. Real-time quantitative PCR analyses of mRNA extracts obtained from purified subpopulations of the SVZ and prostate tumour tissues of DCX-creERT2;loxp-eYFP Hi-MYC mice. The LineYFP+SCA-1PSA-NCAM cells from the SVZ express GFAP and GLAST, which are specific markers of neural stem cells. By contrast, LineYFP+SCA-1PSA-NCAM cells in tumours do not express the stem-cell markers. Three independent experiments. Data are mean + s.e.m.

Source data

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