Extended Data Fig. 5: Genetic lineage tracing of Lin−eYFP+ progenitors during prostate development. | Nature

Extended Data Fig. 5: Genetic lineage tracing of LineYFP+ progenitors during prostate development.

From: Progenitors from the central nervous system drive neurogenesis in cancer

Extended Data Fig. 5

a, After tamoxifen-induced recombination of DCX-creERT2;loxp-eYFP Hi-MYC cancer mice at week 3 after birth, LineYFP + neural progenitors are detected in tumour tissues during the early phases of cancer development, without infiltrating healthy tissues (n = 73), such as testis (n = 34), epididymis (n = 35) and cremaster (n = 29), that surround the tumour. Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Twelve independent experiments. Sample sizes per group are listed in Source Data. b, FACS plots of LineYFP+ cells from the prostate tumour of 6- or 16-week-old Hi-MYC mice. c, Oscillation of LineYFP+ neural progenitors during prostate tumour development in the SVZ (left, n = 163) and olfactory bulbs (middle, n = 159), but not in the dentate gyrus (right, n = 59) of Hi-MYC cancer mice. Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Forty-six independent experiments. Sample sizes per group are listed in Source Data. d, Further monitoring shows marked oscillations of LineYFP+SCA-1PSA-NCAMCD24EGFR cells present in the olfactory bulbs, as observed in SVZ in Fig. 4b. Data are mean + s.e.m. Student;s t-test (one-sided, no adjustment). Forty-six independent experiments. Sample sizes are listed in Source Data. eg, Tamoxifen is not cytotoxic to prostate or brain tissues, as shown by the fact that it does not affect the development of tumours in the Hi-MYC mouse model of cancer (e) and does not alter the cellularity (top) and viability (bottom) of cells in SVZ (f) or olfactory bulbs (g) over time. Data are mean + s.e.m. Student’s t-test (one-sided, no adjustment). Sample sizes are listed in Source Data. *P < 0.05, **P < 0.01, ***P < 0.001.

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