a, Sorting strategy to isolate wild-type epidermal cells. b–f, Principal component analysis (b, via the Jackstraw Method, see Methods) and t-SNE clustering (c). Analysis was performed to confirm that neither cell cycle differences (d) nor batch effects (e) account for the clustering. f, Further subclustering of E17.5 cells reveals distinct lineage populations: interfollicular epidermis (IFE), hair follicle (HF), and differentiating suprabasal cells (SB). Analysis was performed on 227 cells (111 E17.5 cells and 116 E12.5 cells). Heatmap shows the top 30 enriched genes that define each cluster.