Fig. 1: Single-cell RNA sequencing of in vitro β-cell differentiation. | Nature

Fig. 1: Single-cell RNA sequencing of in vitro β-cell differentiation.

From: Charting cellular identity during human in vitro β-cell differentiation

Fig. 1

a, Summary of cell populations identified by flow cytometry at the end of stages 3–6 of the SC-β-cell protocol described in ref. 1. b, Use of inDrops to sample cells from several time points of the same differentiation. c, Expression profiles of developmentally relevant genes and markers across cell types identified in SC-β-cell differentiation. The shading displays mean expression as z-normalized transcripts per million mapped reads (z-norm. TPM), and diameter denotes fractional expression. dg, t-SNE projections of cells sampled from the end of stages 3 through to stage 6 of protocol x1 (see Extended Data Table 1 for definition of protocols). Cells are coloured according to their assigned cluster. Horizontal bars indicate cell-type proportions.