Extended Data Fig. 8: p38γ expression is necessary for Snu398 and HepG2 proliferation. | Nature

Extended Data Fig. 8: p38γ expression is necessary for Snu398 and HepG2 proliferation.

From: p38γ is essential for cell cycle progression and liver tumorigenesis

Extended Data Fig. 8

a, Immunoblot analysis of Snu398 cells treated with lentiviral particles containing two p38γ-targeting shRNAs (B5 or G1) or shScramble. Representative western blot of at least three independent experiments. b, Growth of Snu398 cells infected with shp38γ or shScramble. Cells were plated and cultured for 2 days in medium supplemented with different serum concentrations. Relative cell numbers were measured by crystal violet staining. Data are mean ± s.e.m. (n = 12). Comparisons were made by two-way ANOVA; ***P < 0.001. c, Colony-formation assay of Snu398 cells infected with shp38γ or shScramble. Representative images are shown. d, Cells were grown in DMEM with 10% serum. The number of colonies with >10 cells was counted after 15 days. Data are mean ± s.e.m. (n = 3) and are representative of results from three independent experiments. Comparisons were made by two-sided Student’s t-test; **P < 0.01; ***P < 0.001. e, Immunoblot analysis in HepG2 cells treated with lentiviral particles containing an shRNA against p38γ or Scramble control. Representative western blot of at least three independent experiments. f, Growth of HepG2 cells infected with shp38γ or shScramble. Cells were plated and cultured for 2 days in medium supplemented with different serum concentrations. Relative cell numbers were measured by crystal violet staining. Data are mean ± s.e.m. (n = 3) and are representative of results from three independent experiments. Comparisons were made by two-way ANOVA; ***P < 0.001. g, Left, colony-formation assay of HepG2 cells infected with shp38γ or shScramble. Representative images are shown. Cells were grown in DMEM with 10% serum. Right, the number of colonies with >10 cells was counted after 15 days. Data are mean ± s.e.m. (n = 3). Comparisons were made by two-sided Student’s t-test; ***P < 0.001. h, Soft agar assay of HepG2 cells infected with shp38γ or shScramble. Cells were grown in DMEM with 10% serum. The number of colonies with >10 cells was counted after 20 days. Data are mean ± s.e.m. (n = 12). Comparisons were made by Mann–Whitney U-test; ***P < 0.001.

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