Extended Data Fig. 10: Downregulation of SOAT1 suppresses the proliferation and migration of HCC cells by reducing plasma-membrane cholesterol content, and then inhibiting the integrins and TGFβ signalling pathway. | Nature

Extended Data Fig. 10: Downregulation of SOAT1 suppresses the proliferation and migration of HCC cells by reducing plasma-membrane cholesterol content, and then inhibiting the integrins and TGFβ signalling pathway.

From: Proteomics identifies new therapeutic targets of early-stage hepatocellular carcinoma

Extended Data Fig. 10

a, Western blot of SOAT1 and SOAT2 in PLC/PRF/5 and HepG2 cell lines, with or without treatment with shRNA (n = 3 biologically independent experiments). For full scans, see Supplementary Fig. 1. b, c, SOAT1 protein abundance in PLC/PRF/5 and HepG2 cells based on proteomic data (b, mean ± s.e.m., n = 3 biological repeats) and western blotting analysis (c, n = 3 biologically independent experiments). For full scans, see Supplementary Fig. 1. d, Alteration pathways and proteins in the PLC/PRF/5 cell line after the SOAT1 knockdown or treatment with avasimibe (n = 3 biological repeats). e, Schematic of mechanism that underlies the dysregulation of integrins and TGFβRs by SOAT1 inhibition. f, Body weight of PDX models treated by control (100 μl PBS) or avasimibe (15 mg kg−1 day−1) on the indicated days (n = 36 mice per group). Mean ( ± s.d.) of body weight is plotted. g, Growth curves of PDX models treated by control (100 μl PBS) or avasimibe (15 mg kg−1 day−1) on the indicated days (n = 6 mice per group). Circle and triangle denotes the mean volume of tumours ( ± s.e.m.). The immunohistochemical staining of SOAT1 in PDX models is labelled on the top panel. h, Relative expression of S-III-signature pathway-related proteins among three SOAT1high (SOAT1-H) PDX and three SOAT1low (SOAT1-L) PDX models. Red and blue denote upregulation and downregulation, respectively. Zigzags on the y axis indicate places at which the y axis is truncated. The P values are calculated by two-tailed Student’s t-test. NS, not significant, *P < 0.05, ***P < 0.001.

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