The human genome contains approximately 20 thousand protein-coding genes1, but the size of the collection of antigen receptors of the adaptive immune system that is generated by the recombination of gene segments with non-templated junctional additions (on B cells) is unknown—although it is certainly orders of magnitude larger. It has not been established whether individuals possess unique (or private) repertoires or substantial components of shared (or public) repertoires. Here we sequence recombined and expressed B cell receptor genes in several individuals to determine the size of their B cell receptor repertoires, and the extent to which these are shared between individuals. Our experiments revealed that the circulating repertoire of each individual contained between 9 and 17 million B cell clonotypes. The three individuals that we studied shared many clonotypes, including between 1 and 6% of B cell heavy-chain clonotypes shared between two subjects (0.3% of clonotypes shared by all three) and 20 to 34% of λ or κ light chains shared between two subjects (16 or 22% of λ or κ light chains, respectively, were shared by all three). Some of the B cell clonotypes had thousands of clones, or somatic variants, within the clonotype lineage. Although some of these shared lineages might be driven by exposure to common antigens, previous exposure to foreign antigens was not the only force that shaped the shared repertoires, as we also identified shared clonotypes in umbilical cord blood samples and all adult repertoires. The unexpectedly high prevalence of shared clonotypes in B cell repertoires, and identification of the sequences of these shared clonotypes, should enable better understanding of the role of B cell immune repertoires in health and disease.
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Sequencing data for HIP and CORD datasets have been deposited in the NCBI Sequence Read Archive under project number PRJNA511481. FASTA files for Adaptive Biotechnologies datasets used for analyses are available from https://github.com/crowelab/PyIR. Any other relevant data are available from the corresponding author upon reasonable request.
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We thank M. Mayo and A. Pruijssers for regulatory and human subjects support; G. Sapparapu and O. Koues for technical help; Y. Umareddy for assistance with R; S. B. Day for assistance with artwork; scientists at the VANTAGE core of Vanderbilt University Medical Center (VUMC), Adaptive Biotechnologies, the Genomic Services Laboratory at the Hudson Alpha Institute for Biotechnology, and D. Zhang and team at Abhelix; New England BioLabs for early access to pre-release Abseq reagents; K. Trochez and J. Janssen of the Clinical Trials Center at VUMC and staff and physicians of the Vanderbilt University Medical Center leukapheresis clinic for assistance with large-scale human cell collections; and S. Mallal and M. Pilkinton (Vanderbilt), R. Scheuermann (JCVI), and W. Koff, T. Schenkelberg and the Advisory Board of the Human Vaccines Project for helpful discussions. This work was conducted in part using the resources of the Advanced Computing Center for Research and Education (ACCRE) at Vanderbilt University and the San Diego Supercomputer Center at the University of California, San Diego. We acknowledge the use of cord blood cells procured by the National Disease Research Interchange (NDRI) with support from NIH grant U42 OD11158. This work was supported by a grant from the Human Vaccines Project, and institutional funding from Vanderbilt University Medical Center.
Nature thanks R. Arnaout, F. Breden, A. McHardy and the other anonymous reviewer(s) for their contribution to the peer review of this work.
J.E.C. has served as a consultant for Sanofi and Pfizer, is on the Scientific Advisory Boards of CompuVax and Meissa Vaccines, is a recipient of research grants from Takeda, Sanofi and Moderna, and is founder of IDBiologics. All other authors declare no conflicts of interest.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data figures and tables
Extended Data Fig. 1 Repertoire properties for immunoglobulin V3J clonotype data belonging to HIP1–HIP3.
a, Normalized frequency histogram of HCDR3 sequence lengths belonging to immunoglobulin heavy-chain V3J clonotypes for HIP1 (left, n = 8,623,076 unique HCDR3s, with a median length of 16 amino acids), HIP2 (middle, n = 15,413,214 unique HCDR3s, with a median length of 16 amino acids) and HIP3 (right, n = 7,081,314 unique HCDR3s, with a median length of 15 amino acids). b, Normalized frequency histogram of germline divergence values for HIP1 (left), HIP2 (middle) and HIP3 (right). Germline divergence was defined as 100 per cent minus the per cent nucleotide identity that a read had with its closest matching germline variable (V) gene sequence. Median per cent germline divergence values for HIP1, HIP2 and HIP3 were 3, 0 and 2, respectively. c, Normalized frequency histogram of germline divergence values by isotype for HIP1 (left), HIP2 (middle) and HIP3 (right). The median germline divergence was 0 for all IgM datasets. All isotype data were obtained from the AbHelix sequencing method. d, Heat map representation of unique VH + JH recombinations in HIP1, HIP2 and HIP3. The data from each set were transformed to obtain z-scores, using the mean and s.d. In this figure, the IGH prefix is omitted from the gene symbols for V and J genes. Source data
a, Normalized frequency histogram of HCDR3 sequence lengths belonging to V3J clonotypes from HIP1+2+3all (blue filled curve, n = 30,156,947 unique HCDR3s, with a median length of 16 amino acids) and HIP1+2+3shared (grey bins, n = 22,934 unique HCDR3s, with a median length of 13 amino acids). Medians were statistically different, based on a two-tailed Mann–Whitney U-test with a P < 2.2 × 10−16 (at an α = 0.05). b, Normalized frequency histogram of HCDR3 lengths belonging to all V3DJ clonotypes from HIP1 (n = 1,750,325 unique HCDR3s, with a median length of 19 amino acids), HIP2 (n = 3,889,527 unique HCDR3s, with a median length of 19 amino acids) and HIP3 (n = 1,437,339 unique HCDR3s, with a median length of 19 amino acids). c, Cumulative distribution of normalized VDJ triple frequencies used for simulation. HIP1, n = 4,371 unique VDJ triples; HIP2, n = 4,346 unique VDJ triples; and HIP3, n = 4,370 unique VDJ triples. d, log–log frequency plot between experimental and synthetic HCDR3 lengths. The Pearson correlation coefficient r = 1.00 with a P < 2.2 × 10−16 (at an α = 0.05) (n = 26 CDR3 length bins for each set). e, Normalized frequency histogram of V3DJ overlap counts between all three synthetic HIP distributions (n = 3,641 common clonotypes between sequenced repertoires). f, V3J clonotypes with the largest numbers of somatic variants. Numbers in parentheses denote counts for the number of unique somatic variants associated with a V3J clonotype for HIP1, HIP2 and HIP3. g, Percentage overlaps for the Igκ V3J clonotypes from the experimentally determined repertoires belonging to HIP1–HIP3. h, Percentage overlaps for Igλ V3J clonotypes from the experimentally determined repertoires belonging to HIP1–HIP3. Source data
a, V3DJ clonotype overlaps from three cord blood samples, CORD1 (n = 40,480 unique V3DJ clonotypes), CORD2 (n = 66,718 unique V3DJ clonotypes) and CORD3 (n = 105,555 unique V3DJ clonotypes). b, Cumulative distribution of normalized VDJ triple frequencies for CORD1 (n = 2,273 unique VDJ triples), CORD2 (n = 2,788 unique VDJ triples) and CORD3 (n = 3,002 unique VDJ triples). c, log–log frequency plot between experimental and synthetic CDR3 lengths. The Pearson correlation coefficient r = 1.00 with a P < 2.2 × 10−16 (at an α = 0.05) (n = 21 bins for each set). It should be noted that there were no V3DJ clonotypes with HCDR3s that were less than eight amino acids in length. d, Normalized frequency histogram of V3DJ overlap counts between all three synthetic CORD distributions (n = 45 common clonotypes between all three sequenced repertoires). e, V3J clonotypes identified in HIP1, HIP2 and HIP3 (HIP1+2+3all) were combined with an independently derived set of immunoglobulin heavy-chain V3J clonotypes for which sequences were publicly available7. Starting from the combined set of 59,193,994 clonotypes from six adult immunoglobulin heavy-chain repertoires, each of the three cord blood sets was scanned in a serial fashion, and only the common clonotypes were kept. A total of 130 shared V3J clonotypes was identified. Source data
The flow chart shows how a typical sequencing run using paired-ends reads from Illumina was processed using the bioinformatics pipeline. Detailed descriptions for each of the programs used in the pipeline can be found in Supplementary Methods.
Annotated example of a biological sequence obtained from the two-step barcoded library preparation protocol. The red and yellow regions show the placement of the first and second steps of PCR amplification. The cyan region shows the location of the RID-tagged reverse transcription gene-specific primer.
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Soto, C., Bombardi, R.G., Branchizio, A. et al. High frequency of shared clonotypes in human B cell receptor repertoires. Nature 566, 398–402 (2019). https://doi.org/10.1038/s41586-019-0934-8
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