Extended Data Fig. 4: Single-unit recording of MD. | Nature

Extended Data Fig. 4: Single-unit recording of MD.

From: Neural circuits underlying a psychotherapeutic regimen for fear disorders

Extended Data Fig. 4

a, Coronal section showing the position of the recording sites (red arrow). HB, habenular nucleus; PVT, paraventricular thalamic nucleus. b, c, An example spike sorting result from a single tetrode. b, Example feature plot showing clusters of candidate spikes; c, average waveforms of isolated units from the tetrode. d, Tetrode tip locations in MD. e, f, Positive responses of MD neurons in CS group (e; n = 49 cells) and ABS + CS group (f; n = 63 cells) g, Averaged positive responses across extinction trials (early, second-to-fifth trials; mid, sixth-to-tenth trials; late, eleventh-to-fifteenth trials; samples from e, f). Mixed-design ANOVA: F1,110 = 17.83, P = 4.99 × 10−5 for group effect. h, i, Negative responses of MD neurons in CS group (h; n = 31 cells) and ABS + CS group (i; n = 44 cells) during fear extinction. j, Averaged negative responses of the MD across extinction trials (samples from h, i). Mixed-design ANOVA: F1,73 = 1.762, P = 0.188 for group effect. Mean ± s.e.m.; post hoc multiple comparison with Bonferroni correction; ***P < 0.001. See Supplementary Table 1 for statistical details.