Extended Data Fig. 2: Single-unit recording of SC. | Nature

Extended Data Fig. 2: Single-unit recording of SC.

From: Neural circuits underlying a psychotherapeutic regimen for fear disorders

Extended Data Fig. 2

a, Coronal sections showing the positions of the silicon probes (left) and tetrodes (right). SGS, stratum griseum superficiale; SGI, stratum griseum intermediale; SGP, stratum griseum profundum. b, Schematic of 64-channel silicon probes used for SC recordings. c, Example waveforms of recorded neurons from a single shank. d, Probe tracks (left) and tetrode tip locations (right). e, Example single-unit responses of the SC to sensory stimulation (500-ms bins; pie charts, n = 109 cells). Sensory stimulation blocks were pseudo-randomly presented. f, Averaged SC responses during 5 s after stimulus onset (n = 109 cells). Mixed-design ANOVA: F3,324 = 15.4, P = 2.17 × 10−19 for stimulation effect. g, h, Positive responses of SC neurons from CS group (g; n = 33 cells) and ABS + CS group (h; n = 62 cells) during fear extinction. i, Averaged positive responses across extinction trials (early, second-to-fifth trials; mid, sixth-to-tenth trials; late, eleventh-to-fifteenth trials; samples from g, h). Mixed-design ANOVA: F1,93 = 7.621, P = 0.00695 for group effect. j, k, Negative responses of SC neurons from CS group (j; n = 10 cells) and ABS + CS group (k; n = 8 cells) during fear extinction. l, Averaged negative responses across extinction trials (samples from j, k). Mixed-design ANOVA: F1,16 = 0.71, P = 0.412 for group effect. Mean ± s.e.m.; post hoc multiple comparison with Bonferroni correction; *P < 0.05. See Supplementary Table 1 for statistical details.