Extended Data Fig. 6: Plcb4 deletion disturbing MD activity blocks the effects of ABS paired extinction. | Nature

Extended Data Fig. 6: Plcb4 deletion disturbing MD activity blocks the effects of ABS paired extinction.

From: Neural circuits underlying a psychotherapeutic regimen for fear disorders

Extended Data Fig. 6

a, Effects of the Plcb4 knockout (KO) on ABS paired extinction (wild-type (WT) CS, n = 5; WT ABS + CS, n = 5; KO CS n = 5; KO ABS + CS n = 7 mice). Mixed-design ANOVA for fear extinction: F3,18 = 57.56, P = 2.01 × 10−9 for group effect. One-way ANOVA for recall test: F3,18 = 35.24, P = 9.6 × 10−8. b, Effects of Plcb4 knockdown in MD on ABS paired extinction (shControl CS, n = 4; shControl ABS + CS, n = 7; shPlcb4 CS, n = 4; shPlcb4 ABS + CS, n = 5 mice). Mixed-design ANOVA for fear extinction: F3,16 = 19.25, P = 1.47 × 10−5 for group effect. One-way ANOVA for recall test: F3,16 = 26.18, P = 2.07 × 10−6. Mean ± s.e.m; ***P < 0.001. See Supplementary Table 1 for statistical details. cj, Knockdown of Plcb4 in the MD by injection of shRNA lentiviral vector. Double fluorescence labelling of PLCβ4 expression with DAPI counterstain in the MD of shControl-injected mice (cf) and shPlcb4-injected mice (gj). Histology was confirmed for all mice in b after behavioural experiments. df, hj, Higher magnification images corresponding to the rectangles in c, g, respectively. Scale bars, 1,000 μm (c, g); 100 μm (df, hj).