Extended Data Fig. 3: Production of oxysterols in chondrocytes stimulated with OA-associated catabolic signalling. | Nature

Extended Data Fig. 3: Production of oxysterols in chondrocytes stimulated with OA-associated catabolic signalling.

From: The CH25H–CYP7B1–RORα axis of cholesterol metabolism regulates osteoarthritis

Extended Data Fig. 3

a, b, Chondrocytes were treated with IL-1β or TNFα, or infected with 800 MOI of Ad-C, Ad-CH25H, Ad-CYP7B1, Ad-HIF-2α or Ad-ZIP8. The amounts of 25-HC (a) and 7α,25-HC (b) were measured using HPLC–MS/MS (n = 4 biologically independent samples). Representative peak areas of endogenous oxysterols and corresponding internal standards are shown.

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