Extended Data Fig. 1: Inorganic nitrogen sources are not precursors to SZN. | Nature

Extended Data Fig. 1: Inorganic nitrogen sources are not precursors to SZN.

From: An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin

Extended Data Fig. 1

a, The results of previous feeding experiments suggested that d-glucosamine, l-citrulline or l-arginine, and l-methionine-derived SAM would be building blocks for SZN biosynthesis9. b, Mass spectra of culture extracts in which 15N-nitrate, 15N-nitrite or 15N-ammonium chloride were fed to S. achromogenes var. streptozoticus NRRL 2697. The expected masses ([M + H]+) for SZN, [15N]SZN, [15N2]SZN and [15N3]SZN are 266.0983, 267.0953, 268.0923 and 269.0894, respectively. These results contrast sharply with the strong labelling (>75%) observed in studies of pathways that use nitrite for diazo biosynthesis37,38.

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