Extended Data Fig. 5: Cryo-EM of RP2 class. | Nature

Extended Data Fig. 5: Cryo-EM of RP2 class.

From: Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding

Extended Data Fig. 5

a, Angular distribution calculated in cryoSPARC for particle projections. Heat map shows number of particles for each viewing angle. b, Gold-standard FSC41, calculated by comparing the two independently determined half maps from cryoSPARC. The dotted line represents the 0.143 FSC cut-off, which indicates a nominal resolution of 3.9 Å. c, FSC calculated between the refined structure and the half map used for refinement (work, red), the other half map (free, blue), and the full map (black). d, Top left, the 3.9 Å-resolution cryo-EM density map of RP2. Top right, cross-section of the structure, showing the DNA inside the RNAP cleft. Bottom, same views as above, but coloured by local resolution35.