Extended Data Fig. 5: Immunogenicity of Neo-2/15 in healthy naive mice. | Nature

Extended Data Fig. 5: Immunogenicity of Neo-2/15 in healthy naive mice.

From: De novo design of potent and selective mimics of IL-2 and IL-15

Extended Data Fig. 5

a, Naive C57BL/6 mice were treated daily with Neo-2/15 (n = 10), KO Neo-2/15 (n = 5), mouse IL-2 (n = 5) or left untreated (n = 5). Blood was collected after 28 days and the serum was diluted 1:100 and analysed for IgG against Neo-2/15, mouse IL-2, human IL-2, KO Neo-2/15 and ovalbumin using ELISA. FBS (10%) was used as a negative control. Polyclonal antibody against Neo-2/15 was used as a positive control. All statistical comparisons between sera from treated mice and negative control serum were not significant (two-way ANOVA with a 95% confidence interval). All statistical comparisons between Neo-2/15 and mouse IL-2 treated mice serum were not significant (two-way ANOVA with a 95% confidence interval). The experiments were performed once. b, After 14 days, immune cell populations in the blood of treated mice were quantified by flow cytometry. B cell:T cell ratio (top right) was calculated by dividing the percentage of B220+ cells by the percentage of CD3+ cells. CD8+ cell:CD4+ cell ratio (top left) was calculated by dividing the percentage of CD3+CD8+ cells by the percentage of CD3+CD4+ cells. NK cells (bottom left) were identified by their expression of NK1.1. Results were analysed by one-way ANOVA (95% confidence interval). The experiments were performed once. In all cases, data are mean ± s.d.