Extended Data Fig. 1: Therapeutic effect of Neo-2/15 on colon cancer. | Nature

Extended Data Fig. 1: Therapeutic effect of Neo-2/15 on colon cancer.

From: De novo design of potent and selective mimics of IL-2 and IL-15

Extended Data Fig. 1

a, BALB/C mice were inoculated with CT26 tumours. Starting on day 9 and ending on day 14, mice were treated daily with intraperitoneal injection of mouse IL-2 or Neo-2/15 at the specified concentrations (n = 4 per group), or were left untreated (n = 6 per group). Top, tumour growth curves show data only for surviving mice and stop if the number of mice in a group fell below 50% of the initial number. Bottom, survival curves. Mice were euthanized when weight loss exceeded 10% of initial weight or when tumour size reached 1,300 mm3. The experiments were performed twice with similar results. bd, Bar plots comparing the T cell populations in BALB/C mice (n = 3 per group) that were inoculated with CT26 tumours and treated, starting from day 6, by daily intraperitoneal injection of 10 μg Neo-2/15, 10 μg mouse IL-2 or no treatment (no tx). On day 14 the percentage of Treg cells (CD4+CD45+FoxP3+, top) and CD8:Treg cell ratio (CD45+CD3+CD8+ cells:Treg cells; bottom) were assessed in tumours (b), neighbouring inguinal lymph node (LN) (c), and spleen (d). Data are mean ± s.d., except in growth curves, where data are mean ± s.e.m. Results were analysed by one-way ANOVA (95% confidence interval), except for survival curves that were assessed using the Mantel–Cox test (95% confidence interval). Experiments were performed twice with similar results.