Extended Data Fig. 6: Kinetics of STAT5 phosphorylation with Neo-2/15 treatment. | Nature

Extended Data Fig. 6: Kinetics of STAT5 phosphorylation with Neo-2/15 treatment.

From: De novo design of potent and selective mimics of IL-2 and IL-15

Extended Data Fig. 6

Naive C57BL/6 mice were treated once with 13 µg mouse IL-2 (n = 5) or 10 µg Neo-2/15 (n = 5), or were left untreated (n = 5). Phosphorylation of STAT5 was measured in peripheral blood at the indicated time points by flow cytometry using an anti-pSTAT5 antibody. Mean fluorescence intensity (MFI) is shown at each time point for TCRβ+CD8+ cells (top) and TCRβB220+ cells (bottom). Data are mean ± s.d. Results were analysed by one-way ANOVA (75% confidence interval). The experiments were performed once.