Extended Data Fig. 3: Thermal and chemical denaturation of DHDs. | Nature

Extended Data Fig. 3: Thermal and chemical denaturation of DHDs.

From: Programmable design of orthogonal protein heterodimers

Extended Data Fig. 3

a, b, CD spectra for thermal denaturation of DHD_15 and DHD_20, respectively. Top, wavelength scan at 25 °C, 75 °C, 95 °C and final 25 °C. Designs were α-helical and stable up to 95 °C. Bottom, CD temperature melts, monitoring absorption at 222 nm as temperature was increased from 25 °C to 95 °C. c, GdnHCl denaturation of DHD_127 measured by CD monitoring absorption at 222 nm. All CD experiments were performed once.