Extended Data Fig. 10: Effects of SCH23390 and 1 Hz in vivo in persevering mice. | Nature

Extended Data Fig. 10: Effects of SCH23390 and 1 Hz in vivo in persevering mice.

From: Stochastic synaptic plasticity underlying compulsion in a model of addiction

Extended Data Fig. 10

a, Average traces of EPSCs recorded to determine PPR, rectification index and AMPAR/NMDAR ratio without pharmacological isolation. Ex vivo measurement of the PPR, rectification index and AMPAR/NMDAR ratio after in vivo stimulation of OFC–striatum terminals at 1 Hz for 5 min in persevering mice, in the presence or absence of SCH23390 (ANOVA followed by two-sided t-test: for PPR: t32 = 1.10, P = 0.83; t39 = 0.64, P > 0.99; and t43 = 0.57, P > 0.99 for control versus 1 Hz (n = 19 and 15 cells, respectively); 1 Hz versus 1 Hz with SCH23390 (n = 15 and 26 cells, respectively); and control versus 1 Hz with SCH23390 (n = 19 and 26 cells, respectively); for rectification index: t27 = 0.74, P > 0.99; t39 = 0.17, P > 0.99; and t38 = 1.00, P = 0.97 for control versus 1 Hz (n = 14 and 15 cells, respectively); 1 Hz versus 1 Hz with SCH23390 (n = 15 and 26 cells, respectively); and control versus 1 Hz with SCH23390 (n = 14 and 26 cells, respectively); for AMPAR/NMDAR ratio: t27 = 1.64, P = 0.32; t39 = 5.93, P < 0.0001; and t38 = 3.96, P = 0.0007 for control versus 1 Hz (n = 14 and 15 cells, respectively); 1 Hz versus 1 Hz with SCH23390 (n = 15 and 26 cells, respectively); and control versus 1 Hz with SCH23390 (n = 14 and 26 cells, respectively)). b, Delay to engage the next action was not changed (ANOVA followed by two-sided paired t-test: *P < 0.05 when comparing control versus 1 Hz or control versus 1 Hz with SCH23390, n = 10 mice). oDASS rate was not modified by 1 Hz or 1 Hz with SCH23390 prior to a baseline session (t5 = 0.22, P = 0.84, n = 6 mice for control versus 1 Hz before a baseline session and t9 = 1.48, P = 0.17, n = 10 mice for control versus 1 Hz with SCH23390 before a baseline session). Data are mean ± s.e.m. See Supplementary Table 1 for complete statistics.

Back to article page