a, b, Transient co-expression in tobacco leaf epidermis of POLAR–mCherry with the group-I GSK3/SHAGGY-like kinases ATSK11(ASKα)–GFP, ATSK12(ASKγ)–GFP, and ATSK13(ASKε)–GFP (a) and with the group-II GSK3/SHAGGY-like kinases BIN2(ASKη)–GFP, BIL2(ASKι)–GFP, and (BIL1)ASKζ–GFP (b) leading to their nuclear exclusion. c, Transient co-expression of POLAR–mCherry with the group-III GSK3/SHAGGY-like kinases ATSK32(ASKθ)–GFP and ATSK31(ASKβ)–GFP. ATSK31(ASKβ)–GFP was excluded from the nucleus, whereas ATSK32(ASKθ)–GFP was not; POLAR–mCherry was included into the nucleus with ATSK32(ASKθ)–GFP. d, Transient co-expression of POLAR–mCherry with the group-IV GSK3/SHAGGY-like kinases ATSK41(ASKκ)–GFP and ATSK42(ASKδ)–GFP. When expressed alone, ATSK41(ASKκ)–GFP and ATSK42(ASKδ)–GFP did not localize to the nucleus, and coinfiltration with POLAR–mCherry did not affect their localization. White arrowheads in a–d indicate the nucleus. For a-d, experiments were repeated independently three times. Scale bars, 20 µm.