Extended Data Fig. 9: Stomatal phenotypes in abaxial epidermis of cotyledons at 21 d.p.g. | Nature

Extended Data Fig. 9: Stomatal phenotypes in abaxial epidermis of cotyledons at 21 d.p.g.

From: POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Extended Data Fig. 9

a, Wild types (Col-0 or Wassilewskija (Ws)), polar-2, pl1-1, polar-2 pl1-1, polar-3 (CRISPR), pl1-2 (CRISPR), polar-4 pl1-2 (CRISPR), POLAR:POLAR-GFP;Col-0 (line 14.5), bin2-3, atsk12, bin2-3 atsk12, bin2-3 bil1 bil2 atsk13-RNAi, TMM:BIN2-GSgreen;bin2-3 (line 17.6), TMM:BIN2-GSgreen;Col-0 (line 6.2), TMM:BIN2-GSgreen;POLAR:POLAR-mCherry (line 6), and basl-2 seedlings. Scale bar, 100 µm. b, Pavement cell size distribution of genotypes described in a with significant phenotypes. n, number of cells from three biologically independent cotyledons. c, Quantification of the pavement cell density, stomatal density, total cell density, the stomatal index, and the stomatal clustering index in the genotypes described in a. n, number of biologically independent cotyledons. Box plots show the first and third quartiles, split by the median (line) and mean (cross). Statistical analysis was done with a one-way ANOVA and Tukey’s post hoc test. All values were compared to Col-0 unless indicated with brackets. The bin2-3 bil1 bil2 atsk13-RNAi quadruple mutant was compared to Ws. For ac, experiments were repeated independently three times.

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