a, Confocal images of abaxial epidermis of cotyledons of seedlings at 3 d.p.g. expressing group-I ATSK11:ATSK11-GFP;Col-0 (AT5G26751), ATSK12:ATSK12-GFP;Col-0 (AT3G05840) or ATSK13:ATSK13-GFP (AT5G14640) and group-II BIN2:BIN2-GFP;Col-0, BIL2:BIL2-GFP;Col-0 (AT1G06390) and BIL1:BIL1-GFP;Col-0 (AT2G30980). ATSK11–GFP, ATSK12–GFP, ATSK13–GFP, BIN2–GFP, BIL2–GFP and BIL1–GFP proteins showed stabilization in the stomatal lineage and polarization during ACD, with group-I members displaying a more frequent polarization. b, Co-expression of all BIN2 homologues with POLAR–mCherry leading to their stabilization and apparent polarization. White arrowheads in a and b indicate stabilization and/or polarization and correspond to insets in every picture. c, Co-expression of BIN2 with mCherry–BASL did not lead to BIN2 stabilization and polarization. All experiments were repeated independently three times. Scale bars, 20 µm.