Extended Data Fig. 3: Tumour cells are arginine auxotrophs. | Nature

Extended Data Fig. 3: Tumour cells are arginine auxotrophs.

From: Autophagy maintains tumour growth through circulating arginine

Extended Data Fig. 3

a, YUMM 1.3, 71.8, MB49 and YUMM 1.7, 1.9 proliferation in vitro, in medium containing different percentages of arginine. Cell density was measured every 2 h using the IncuCyte. Data are representative of three independent experiments performed in duplicate. b, Western blotting showing expression of ASS1, ASL and OTC in kidneys and livers from Atg7+/+ and Atg7Δ/Δ hosts. *P < 0.05 compared to Atg7+/+ hosts. Data are representative of three independent experiments. Actin was used as a loading (kidney ASL and liver OTC) and processing (kidney ASS1, liver ASS1 and ASL) control. c, Western blotting showing expression of ASS1, ASL and OTC in YUMM 1.7 tumours from Atg7+/+ and Atg7Δ/Δ hosts. Data are representative of two independent experiments. Actin was used as a loading (OTC) and processing (ASS1 and ASL) control. d, Analysis of levels of nitric oxide in serum in Atg7+/+ (n = 11) and Atg7Δ/Δ (n = 9) hosts. Data are mean ± s.e.m.

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