Abstract

As the first line of defence against pathogens, cells mount an innate immune response, which varies widely from cell to cell. The response must be potent but carefully controlled to avoid self-damage. How these constraints have shaped the evolution of innate immunity remains poorly understood. Here we characterize the innate immune response’s transcriptional divergence between species and variability in expression among cells. Using bulk and single-cell transcriptomics in fibroblasts and mononuclear phagocytes from different species, challenged with immune stimuli, we map the architecture of the innate immune response. Transcriptionally diverging genes, including those that encode cytokines and chemokines, vary across cells and have distinct promoter structures. Conversely, genes that are involved in the regulation of this response, such as those that encode transcription factors and kinases, are conserved between species and display low cell-to-cell variability in expression. We suggest that this expression pattern, which is observed across species and conditions, has evolved as a mechanism for fine-tuned regulation to achieve an effective but balanced response.

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Data availability

Sequencing data have been deposited in ArrayExpress with the following accessions: E-MTAB-5918, E-MTAB-5919, E-MTAB-5920, E-MTAB-6754, E-MTAB-6773, E-MTAB-5988, E-MTAB-5989, E-MTAB-6831, E-MTAB-6066, E-MTAB-7032, E-MTAB-7037, E-MTAB-7051 and E-MTAB-7052.

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Acknowledgements

We thank N. Eling, M. Fumagalli, Y. Gilad, O. Laufman, A. Marcovitz, J. Marioni, K. Meyer, M. Muffato, D. Odom, O. Stegle, A. Stern, M. Stubbington, V. Svensson and M. Ward for discussions; G. Emerton, A. Jinat, L. Mamanova, K. Polanski, A. Fullgrabe, N. George, S. Barnett, R. Boyd, S. Patel and C. Gomez for technical assistance; the Hipsci consortium for human fibroblast lines; and members of the Teichmann laboratory for support at various stages. This project was supported by ERC grants (ThDEFINE, ThSWITCH) and an EU FET-OPEN grant (MRG-GRAMMAR No 664918) and Wellcome Sanger core funding (Grant No WT206194). T.H. was supported by an HFSP Long-Term Fellowship and by EMBO Long-Term and Advanced fellowships. V.P. is funded by Fondazione Umberto Veronesi.

Reviewer information

Nature thanks L. Barreiro, I. Yanai and the other anonymous reviewer(s) for their contribution to the peer review of this work.

Author information

Affiliations

  1. Wellcome Sanger Institute, Cambridge, UK

    • Tzachi Hagai
    • , Xi Chen
    • , Ricardo J. Miragaia
    • , Raghd Rostom
    • , Tomás Gomes
    • , Natalia Kunowska
    • , Johan Henriksson
    • , Jong-Eun Park
    • , Lara Bossini-Castillo
    • , Felipe A. Vieira Braga
    • , Gosia Trynka
    •  & Sarah A. Teichmann
  2. EMBL- European Bioinformatics Institute, Cambridge, UK

    • Tzachi Hagai
    • , Raghd Rostom
    • , Guy Naamati
    •  & Sarah A. Teichmann
  3. Centre of Biological Engineering, University of Minho, Braga, Portugal

    • Ricardo J. Miragaia
  4. Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy

    • Valentina Proserpio
    •  & Giacomo Donati
  5. Italian Institute for Genomic Medicine (IIGM), Torino, Italy

    • Valentina Proserpio
  6. Molecular Biotechnology Center, University of Turin, Torino, Italy

    • Giacomo Donati
  7. Open Targets, Wellcome Sanger Institute, Cambridge, UK

    • Felipe A. Vieira Braga
  8. Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK

    • James Fletcher
    • , Emily Stephenson
    • , Peter Vegh
    •  & Muzlifah Haniffa
  9. Division of Pathology and Microbiology, Animal Science Department, Biomedical Primate Research Centre, Rijswijk, The Netherlands

    • Ivanela Kondova
  10. Research Department, Public Health England, National Infection Service, Porton Down, UK

    • Mike Dennis
  11. Department of Dermatology and NIHR Newcastle Biomedical Research Centre, Newcastle Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK

    • Muzlifah Haniffa
  12. Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany

    • Armita Nourmohammad
  13. Department of Physics, University of Washington, Seattle, WA, USA

    • Armita Nourmohammad
  14. Institute for Biological Physics, University of Cologne, Cologne, Germany

    • Michael Lässig
  15. Theory of Condensed Matter Group, Cavendish Laboratory, University of Cambridge, Cambridge, UK

    • Sarah A. Teichmann

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Contributions

T.H. and S.A.T. designed the project; T.H., X.C., R.J.M., R.R., N.K. and J.-E.P. performed experiments with help from V.P., G.D. and F.A.V.B.; T.H., X.C., R.J.M., R.R., T.G. and J.H. analysed the data with help from G.N., L.B.-C., G.T, A.N. and M.L.; J.F., E.S., P.V., I.K., M.D. and M.H. provided samples; S.A.T. supervised the project; T.H., R.R., N.K. and S.A.T. wrote the manuscript with input from all authors.

Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Tzachi Hagai or Sarah A. Teichmann.

Extended data figures and tables

  1. Extended Data Fig. 1 Fibroblast response to dsRNA and IFNB across species.

    To study the similarity in response to treatment across species, we plotted the fold-change values of all expressed genes (with one-to-one orthologues) between pairs of species (human–macaque, mouse–rat and human–mouse) in response to dsRNA (poly(I:C)) (ac). As a control, we performed the same procedure with IFNB stimulations (df). Fold-changes were inferred from differential expression analyses, determined by the exact test in the edgeR package6 and based on n = 6, 5, 3 and 3 individuals from human, macaque, rat and mouse, respectively. Spearman correlations between all expressed one-to-one orthologues are shown in grey, Spearman correlations between the subset of differentially expressed genes (FDR-corrected P < 0.01 in at least one species) appear in black. Number of genes shown is n = 11,035, 11,005, 11,137, 10,851, 10,826 and 10,957 in af, respectively. Genes are coloured blue if they were differentially expressed (FDR-corrected P < 0.01) in both species, purple if they were differentially expressed in only one species, or red if they were not differentially expressed. g, h, Density plots of ratio of fold-change in response to dsRNA or to IFNB. g, Comparison between human and macaque orthologues in dsRNA response. h, Comparison between human and mouse orthologues in IFNB response. i, Dendrogram based on the fold-change in response to dsRNA or to IFNB across 9,835 one-to-one orthologues in human, macaque, rat and mouse.

  2. Extended Data Fig. 2 Correspondence of transcriptional divergence and divergence of active promoters and enhancers.

    Comparison of divergence in transcriptional response to dsRNA with divergence of active chromatin marks in active promoters (a, profiled using H3K4me3 in proximity to gene’s TSS) and enhancers (b, H3K27ac without overlapping H3K4me3). Chromatin marks were linked to genes on the basis of their proximity to the gene’s TSS. Chromatin marks were obtained from n = 3 individuals in each of the four species, from fibroblasts stimulated with dsRNA or left untreated. The statistics are based on n = 855, 818 and 813 human genes that have a linked H3K4me3 mark with a syntenic region in macaque, rat and mouse, respectively (a); and on n = 326, 241 and 242 human genes that have a linked H3K27ac mark with a syntenic region in macaque, rat and mouse, respectively (b). Each panel shows the fraction of conserved marks between human and macaque, rat or mouse, in genes that have high, medium and low divergence in their transcriptional response. In each column, the histone mark’s signal was compared between human and the syntenic region in one of the three other species. If an active mark was found in the corresponding syntenic region, the linked gene was considered to have a conserved active mark (promoter or enhancer). The fractions of genes with conserved promoters (or enhancers) in each pair of species were compared between high- and low-divergence genes using a one-sided Fisher’s exact test. When comparing active promoter regions of high- versus low-divergence genes, we observe that low-divergence genes have a significantly higher fraction of conserved marks in rodents. This suggests an agreement between divergence at the transcriptional and chromatin levels in active promoter regions. In active enhancer regions, we do not observe these patterns, suggesting that the major contribution to divergence comes from promoters.

  3. Extended Data Fig. 3 Comparison of response divergence of genes containing various promoter elements.

    Comparison of response divergence between genes with and without a TATA-box and a CGI. Left, fibroblasts (n = 14, 14, 633 and 294 differentially expressed genes with only TATA-box element, with both CGI and TATA-box elements, with only CGI, and with neither element in their promoters, respectively); right, phagocytes (n = 13, 29, 1,718 and 576 differentially expressed genes with only a TATA-box element, with both CGI and TATA-box elements, with only a CGI, and with neither element in their promoters, respectively). Genes with a TATA-box without a CGI have higher response divergence than genes with both elements. Genes with a CGI but without a TATA-box diverge more slowly than genes with both elements. Genes with both elements do not differ significantly in their divergence from genes lacking both elements (one-sided Mann–Whitney test). Data in boxplots represent the median, first quartile and third quartile with lines extending to the furthest value within 1.5 of the IQR.

  4. Extended Data Fig. 4 Response divergence of molecular processes upregulated in immune response.

    Left, distributions of divergence values of n = 955 dsRNA-responsive genes in fibroblasts and subsets of this group belonging to different biological processes. For each functional subset, the distribution of divergence values is compared with the set of 955 dsRNA-responsive genes using a one-sided Mann–Whitney test. FDR-corrected P values are shown above each group and group size is shown inside each box. Right, distributions of divergence values of n = 2,336 LPS-responsive genes in mononuclear phagocytes and subsets of this group belonging to different biological processes. For each functional subset, the distribution of divergence values is compared with the set of 2,336 LPS-responsive genes. FDR-corrected P values (one-sided Mann–Whitney test) are shown above each group and group size is shown inside each box. Data in boxplots represent the median, first quartile and third quartile with lines extending to the furthest value within 1.5 of the IQR.

  5. Extended Data Fig. 5 Cell-to-cell variability versus response divergence across species and conditions in fibroblasts after dsRNA stimulation.

    Cell-to-cell variability values, as measured with DM across individual cells, compared with response divergence between species (grouped into low, medium and high divergence). Variability values are based on n = 29, 56, 55, 35 human cells, n = 20, 32, 29, 13 rhesus cells, n = 33, 70, 65, 40 rat cells, and n = 53, 81, 59, 30 mouse cells, stimulated with dsRNA for 0, 2, 4 and 8 h, respectively. Rows represent different dsRNA stimulation time points (0, 2, 4 and 8 h), and columns represent different species as shown. High-divergence genes were compared with low-divergence genes using a one-sided Mann–Whitney test. Data in boxplots represent the median, first quartile and third quartile with lines extending to the furthest value within 1.5 of the IQR.

  6. Extended Data Fig. 6 Cell-to-cell variability versus response divergence across species and conditions in mononuclear phagocytes after LPS stimulation.

    Cell-to-cell variability values, as measured with DM across cells, compared with response divergence between species (grouped into low, medium and high divergence). Variability values are based on n = 3,519, 4,321, 3,293, 2,126 mouse cells, n = 2,266, 2,839, 1,963, 1,607 rat cells, n = 3,275, 1,820, 1,522, 1,660 rabbit cells, and n = 1,748, 1,614, 1,899, 1,381 pig cells, stimulated with LPS for 0, 2, 4 and 6 h, respectively. Rows represent different LPS stimulation time points (0, 2, 4 and 6 h), and columns represent different species as shown. High-divergence genes were compared with low-divergence genes using a one-sided Mann–Whitney test. Data in boxplots represent the median, first quartile and third quartile with lines extending to the furthest value within 1.5 of the IQR.

  7. Extended Data Fig. 7 Cell-to-cell variability of cytokine expression in single cell in situ RNA hybridization assay combined with flow cytometry (PrimeFlow).

    PrimeFlow measurement of two cytokine genes (IFNB and CXCL10) that show high cell-to-cell variability in scRNA-seq. As controls, two genes matched on expression levels (ATXN2L and ADAM32) but that show low cell-to-cell variability in scRNA-seq data are shown. As the expression of cytokines is at the low end of the distribution, we also chose two genes with middle-range expression values (ADAMTSL3 and BRD2) as additional controls. The experiment was performed in n = 2 independent replicates, originating from the same individual. Both replicates are shown. a, Pseudocolour contour plot for RNA target expression in dsRNA-stimulated human fibroblasts. The x-axis shows area of side scatter (SSC-A) and the y-axis shows fluorescent signal for target RNA probes. RNA targets detected by the same fluorescent channel are displayed together. Top, IFNB and control genes BRD2 and ATXN2L, type 1 probe, Alexa FluorTM 647. Bottom, CXCL10 and control genes ADAMTSL3 and ADAM32, type 10 probe, Alexa FluorTM 568. The cytokine genes display a broader range of fluorescence signal than the controls. b, Histograms comparing fluorescence of cytokine and control pairs (IFNBBRD2 for type 1 probe and CXCL10ADAM32 for type 10 probe). The histograms show a bimodal distribution of expression signal for the two cytokine genes (IFNB and CXCL10, red), but not for controls (blue). This agrees with scRNA-seq data in which CXCL10 and IFNB display high levels of cell-to-cell variability.

  8. Extended Data Fig. 8 Cell-to-cell variability levels and response divergence of cytokines, transcription factors and kinases in response to LPS stimulation of phagocytes.

    A scatter plot showing divergence in response to LPS across species and transcriptional cell-to-cell variability in mouse mononuclear phagocytes following 4 h of LPS treatment, in n = 2,262 LPS-responsive genes. Purple, cytokines; green, transcription factors; beige, kinases. The distributions of divergence values and cell-to-cell variability values of each of the three functional groups are shown above and to the right of the scatter plot, respectively.

  9. Extended Data Fig. 9 Cell-to-cell variability levels in cytokines, transcription factors and kinases across species and stimulation time points.

    Violin plots showing the distribution of cell-to-cell variability values (DM) of cytokines, transcription factors and kinases during immune stimulation. Left, fibroblast dsRNA stimulation time course. Number of cells used in each species (at 2, 4, 8 h dsRNA, respectively): human, 56, 55, 35; macaque, 32, 29, 13; rat, 70, 65, 40; mouse, 81, 59, 30. Right, phagocyte LPS stimulation time course. Number of cells used in each species (at 2, 4, 6 h LPS, respectively): mouse, 4,321, 3,293, 2,126; rat, 2,839, 1,963, 1,607; rabbit, 1,820, 1,522, 1,660; pig, 1,614, 1,899, 1,381. For both panels, colours as in Fig. 3c. Comparisons between groups of genes were performed using one-sided Mann–Whitney tests. Violin plots show the kernel probability density of the data.

  10. Extended Data Fig. 10 Percentage of cells expressing cytokines, transcription factors and kinases.

    Histograms showing the percentage of fibroblasts expressing cytokines (top), transcription factors (middle) and kinases (bottom) following 4 h dsRNA stimulation, in human, macaque, rat and mouse cells (based on n = 55, 29, 65 and 59 cells, respectively). The percentage of expressing cells is divided into 13 bins (x-axis). The y-axis represents the fraction of genes from this gene class (for example, cytokines) that are expressed in each bin (for example, in human, nearly 30% of the cytokine genes (y-axis) are expressed in the first bin, corresponding to expression in fewer than 8% of cells).

Supplementary information

  1. Supplementary Information

    This file contains Supplementary Methods, Analyses and Discussion, Supplementary Tables 1-2, 5-7 and Supplementary Figures 1-17

  2. Reporting Summary

  3. Supplementary Table 3

    GO-term enrichment analysis of genes in dsRNA treatment – see Supplementary Information for full description

  4. Supplementary Table 4

    A list of expressed genes in fibroblasts and phagocytes across species – see Supplementary Information for full description

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https://doi.org/10.1038/s41586-018-0657-2

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