Extended Data Fig. 2: DNA damage induces nuclear translocation of cGAS in lung cancer cells. | Nature

Extended Data Fig. 2: DNA damage induces nuclear translocation of cGAS in lung cancer cells.

From: Nuclear cGAS suppresses DNA repair and promotes tumorigenesis

Extended Data Fig. 2

ae, Immunofluorescence assay results showing localization of transfected HA–cGAS (anti-HA, green) in A549 cells that were untreated (a) or treated with etoposide (100 μg ml−1) (b), camptothecin (1 μM) (c) or H2O2 (10 mM) (d) for the indicated times. Data represent n = 3 independent experiments. Quantitative data are depicted in e. At least 100 transfected cells were counted in each experiment. Data are expressed as mean ± s.e.m. Scale bar, 5 μm. f, g, Immunofluorescence assay results showing localization of transfected HA–cGAS (anti-HA, green) in PC-9 cells that were untreated (NT) or treated with indicated concentrations of etoposide, camptothecin or H2O2 for the indicated times (f). Data represent n = 3 independent experiments. Quantitative data were depicted in g. At least 100 transfected cells were counted in each experiment. Data are expressed as mean ± s.e.m. Scale bar, 5 μm. h, Immunoblot results showing cytoplasmic and nuclear fractions of A549 cells transfected with pcDNA3.1-HA (HA) or pcDNA3.1-HA–cGAS (HA–cGAS) for 48 h and then treated with etoposide (100 μg ml−1) for the indicated times. Data are representative of n = 2 independent experiments. For gel source data, see Supplementary Fig. 1.