Fig. 1: Transcriptional recording by CRISPR spacer acquisition from RNA. | Nature

Fig. 1: Transcriptional recording by CRISPR spacer acquisition from RNA.

From: Transcriptional recording by CRISPR spacer acquisition from RNA

Fig. 1

a, Expression of RT–Cas1–Cas2 leads to the acquisition of intracellular RNAs, providing a molecular memory of transcriptional events stored within DNA. b, Comparison of RNA-seq and Record–seq. RNA-seq captures the transcriptome of a population of cells at a single point in time, providing a transient snapshot of cellular events. By contrast, Record–seq permanently stores information about prior transcriptional events in a CRISPR array, providing a molecular record that can be used to reconstruct transcriptional events that occurred over time.