Extended Data Fig. 4: Differentiation of PSCs, PP1 and PP2 cells when transplanted into kidney capsule of secondary hosts and FACS analysis of CTSK–mGFP calvarial cells. | Nature

Extended Data Fig. 4: Differentiation of PSCs, PP1 and PP2 cells when transplanted into kidney capsule of secondary hosts and FACS analysis of CTSK–mGFP calvarial cells.

From: Discovery of a periosteal stem cell mediating intramembranous bone formation

Extended Data Fig. 4

a, CTSK–mGFP+ PSCs (green) were immunostained for THY1.2 (magenta, top), 6C3 (magenta, middle) and CD105 (magenta, bottom) three weeks after transplantation into the kidney capsule of primary recipients. b, CTSK–mGFP+ PP1 cells (green) were immunostained for 6C3 (magenta, top) and CD105 (magenta, bottom) three weeks after transplantation into the kidney capsule. c, CTSK–mGFP+ PP2 cells (green) were immunostained for THY1.2 (magenta, top), and CD105 (magenta, bottom) three weeks after transplantation into the kidney capsule. Scale bar, 50 µm. Images in ac are representative of 3 independent experiments. Scale bar, 50 µm. d, e, FACS analysis of PSCs, PP1s and PP2s at P15 in mouse calvarial suture (d) and calvarial periosteum (e). f, g, FACS for THY1.2 in CTSK–mGFP cells isolated from calvarial suture ((i), (i)) and calvarial periosteum ((ii), (ii)) at day 15 (left plots) and day 32 (right plots). Plots in dg are representative of results from 3 independent experiments. h, CD146 and SCA1 expression in CTSK–mGFP cells from the suture (i, ii) and periosteum (iii, iv) of P6 mouse calvarium. Representative FACS plots from 10 independent experiments. i, j, FACS for CD146 ((i), (i)) and SCA1 ((ii), (ii)) in calvarial periosteum of mice at day 15 (top plots) and day 32 (bottom plots). Representative FACS plots from 3 independent experiments.

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