Extended Data Fig. 2: Detection of mitotic standard stages. | Nature

Extended Data Fig. 2: Detection of mitotic standard stages.

From: Experimental and computational framework for a dynamic protein atlas of human cell division

Extended Data Fig. 2

a, Detection of major mitotic transitions of the mitotic standard time. Peaks in the second derivatives (red circles) above a pre-defined threshold (grey lines) were detected in all feature dimensions as mitotic transitions. b, Additional smaller peaks (blue dots) were detected to ensure a maximum duration of 12 min for each standard stage. c, Transitions were deleted (grey circles) such that all stages had a minimal duration of 1.5 min. d, The standard mitotic cell was represented by the cell closest to the mean of each stage. Each mitotic stage was assigned duration (coloured line), its duration s.d. (grey line) and a biological annotation.

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